Sleiman Lab Publications

Control of the Assembly and Disassembly of Spherical Nucleic Acids is Critical for Enhanced Gene Silencing

Jathavan Asohan, Hassan H Fakih, Trishalina Das, Hanadi F Sleiman

ACS Nano. 2024, 18, 3996-4007. DOI:10.1002/ange.202217814

DNA-Mediated Peptide Assembly into Protein Mimics

Fangzhou Zhao, Martin Frandsen, Sabrina Capodaglio, Hanadi F Sleiman

J. Am. Chem. Soc. 2024, 146, 3, 1946-1956. DOI:10.1002/ange.202217814

Automated Synthesis of Wireframe DNA Nanotubes

Patricia Islas, Casey M. Platnich, Yasser Gidi, Ryan Karimi, Lorianne Ginot, Gonzalo Cosa, Hanadi F Sleiman

bioRxiv. 2024, 01, 18, 576297. DOI:10.1002/ange.202217814

Impact of the Core Chemistry of Self-Assembled Spherical Nucleic Acids on their In Vitro Fate

Sinan Faiad, Dr. Quentin Laurent, Dr. Alexander L. Prinzen, Jathavan Asohan, Dr. Daniel Saliba. Dr. Violeta Taoder, Prof. Hanadi F. Sleiman

Angew Chem. 2023, 135, 51. DOI:10.1002/ange. 202315768

Dendritic amphiphilic siRNA: Selective albumin binding, in vivo efficacy and low toxicity

Hassan H. Fakih, Qi Tang, Ashely Summers, Minwook Shin, Julianna E. Buchwald, Rosemary Gagnon, Vignesh N. Hariharan, Dimas Echeverria, David A. Cooper, Jonathan K. Watts, Anastasia Khvorova, Hanadi F. Sleiman

Molecular Therapy-Nucleic Acids. 2023, 34, 102080. DOI:10.1016/j.omtn. 2023.102080

Minimalist Design of Wireframe DNA Nanotubes: Tunable Geometry, Size, Chirality, and Dynamics

Xin Luo, Daniel Saliba, Tianxiao Yang, Serena Gentile, Keita Mori, Patricia Islas, Trishalina Das, Neda Bagheri, Alessandro Porchetta, Alba Guarne, Gonzalo Cosa, Hanadi F. Sleiman

Angew. Chem. 2023, 62, 44. DOI:10.1002/anie.202309869

Supramolecular Nucleic Acid-Based Organosilica Nanoparticles Responsive to Physical and Biological Inputs

Pierre Picchetti, Stefano Volpi, Maria Sancho-Albero, Marianna Rossetti, Michael D. Dore, Tuan Trinh, Frank Biedermann, Martin Neri, Alessandro Bertucci, Alessandro Porchetta, Roberto Corradini, Hanadi F Sleiman, Luisa De Cola

J. Am. Chem. Soc. 2023, 145, 42, 22903-22912. DOI:10.1021/jacs.3c04345

Responsive Nucliec Acid-Based Organosilica Nanoparticles

Pierre Picchetti, Stefano Volpu, Marianna Rossetti, Michael D Dore, Tuan Trinh, Frank Biedermann, Martina Neri, Alessandro Bertucci, Alessandro Porchetta, Roberto Corradini, Hanadi Sleiman, Luisa De Cola

J. Am. Chem. Soc. 2023, 145, 42, 22896-22902 DOI:10.1021/jacs.3c00393

Ultrabright DNA nanostructures for biosensing

Xighua Shi, Haidong Huang, Hassan H Fakih, Hanadi F Sleiman

US Patent Application

DNA-encoded functionalized apatamers

Hanadi Sleiman, Donatien Philippe, Marie Lacroix De Vimeur, De Rochame, Maureen McKeague, Serhii Hirka, Daniel Saliba, Shaun Ian Anderson

US Patent Application

Two-Dimensional Supramolecular Polymerization of DNA Amphiphiles is Driven by Sequence-Dependent DNA-Chromophore Interactions

Muhammad Ghufran Rafique, Jacob M Remington, Finley Clark, Haochen Bai, Violeta Toader, Dmytro F Perepichka, Jianing Li, Hanadi F Sleiman

Angew. Chem. 2023, 135-24. DOI:10.1002/ange.202217814

Sequence-Controlled Spherical Nucleic Acids: Gene Silencing, Encapsulation, and Cellular Uptake

Sepideh Kaviani, Hassan H Fakih, Jathavan Asohan, Adam Katolik, Masad J Damha, Hanadi F Sleiman

Nucleic Acid Ther. 2023, 2159-3337. DOI:10.1089/nat.2022.0062

A Single Monomer Difference can Impact the Nanostructure Output of Precision Oligo(phosphodiesters)

Donatien de Rochambeau, Maciej Barłóg, Felix Rizzuto, Quentin Laurent, Xin Luo, Kai Lin Lau, Hassan S Bazzi, Hanadi F Sleiman

Polym. Chem. 2023, 1759-9962. DOI: 10.1039/d3py00731f

2013

A platinum(II) phenylphenanthroimidazole with an extended side-chain exhibits slow dissociation from a c-Kit G-quadruplex motif.

Castor KJ, Liu Z, Fakhoury J, Hancock MA, Mittermaier A, Moitessier N, Sleiman HF.

Chem. Eur. J., 2013 Dec 23;19(52):17836-45.

Site-specific positioning of dendritic alkyl chains on DNA cages enables their geometry-dependent self-assembly.

Edwardson TG, Carneiro KM, McLaughlin CK, Serpell CJ, Sleiman HF.

Nat. Chem. 2013 Oct;5(10):868-75.

Electrogenerated chemiluminescence of iridium-containing ROMP block copolymer and self-assembled micelles.

Tefashe UM, Metera KL, Sleiman HF, Mauzeroll J.

Langmuir, 2013 Oct 15; 29(41):12866-73.

Intercalators as molecular chaperones in DNA self-assembly.

Greschner AA, Bujold KE, Sleiman HF.

J. Am. Chem. Soc. 2013 Jul 31;135(30):11283-8.

Long-range assembly of DNA into nanofibers and highly ordered networks.

Carneiro KM, Avakyan N, Sleiman HF.

Wiley Interdiscip. Rev. Nanomed. Nanobiotechnol. 2013 May-Jun;5(3):266-85.

Simple design for DNA nanotubes from a minimal set of unmodified strands: rapid, room-temperature assembly and readily tunable structure.

Hamblin GD, Hariri AA, Carneiro KM, Lau KL, Cosa G, Sleiman HF.

ACS Nano. 2013 Apr 23;7(4):3022-8.

2012

The role of organic linkers in directing DNA self-assembly and significantly stabilizing DNA duplexes.

Greschner AA, Toader V, Sleiman HF.

J. Am. Chem. Soc. 2012 Sep 5;134(35):14382-9.

Three-dimensional organization of block copolymers on “DNA-minimal” scaffolds.

McLaughlin CK, Hamblin GD, Hänni KD, Conway JW, Nayak MK, Carneiro KM, Bazzi HS, Sleiman HF.

J. Am. Chem. Soc. 2012 Mar 7;134(9):4280-6.

Rolling circle amplification-templated DNA nanotubes show increased stability and cell penetration ability.

Hamblin GD, Carneiro KM, Fakhoury JF, Bujold KE, Sleiman HF.

J. Am. Chem. Soc. 2012 Feb 15;134(6):2888-91. Erratum in: J Am Chem Soc. 2012 Mar 21;134(11):5426.

Platinum(II) phenanthroimidazoles for targeting telomeric G-quadruplexes.

Castor KJ, Mancini J, Fakhoury J, Weill N, Kieltyka R, Englebienne P, Avakyan N, Mittermaier A, Autexier C, Moitessier N, Sleiman HF.

ChemMedChem. 2012 Jan 2;7(1):85-94.

2011

Supramolecular DNA assembly.

McLaughlin CK, Hamblin GD, Sleiman HF.

Chem. Soc. Rev. 2011 Dec;40(12):5647-56. Review.

A facile, modular and high yield method to assemble three-dimensional DNA structures.

McLaughlin CK, Hamblin GD, Aldaye FA, Yang H, Sleiman HF.

Chem. Commun. (Camb). 2011 Aug 21;47(31):8925-7. doi: 10.1039/c1cc11726b. Epub 2011 Jul 11.

Self-assembly of metal-DNA triangles and DNA nanotubes with synthetic junctions.

Yang H, Lo PK, McLaughlin CK, Hamblin GD, Aldaye FA, Sleiman HF.

Methods Mol. Biol. 2011;749:33-47. doi: 10.1007/978-1-61779-142-0_3.

Chiral metal-DNA four-arm junctions and metalated nanotubular structures.

Yang H, Altvater F, de Bruijn AD, McLaughlin CK, Lo PK, Sleiman HF.

Angew. Chem. Int. Ed. Engl. 2011 May 9;50(20):4620-3. doi: 10.1002/anie.201007403. Epub 2011 Apr 26. No abstract available.

2010

Loading and selective release of cargo in DNA nanotubes with longitudinal variation.

Lo PK, Karam P, Aldaye FA, McLaughlin CK, Hamblin GD, Cosa G, Sleiman HF.

Nat. Chem. 2010 Apr;2(4):319-28. doi: 10.1038/nchem.575. Epub 2010 Mar 14.

Self-assembly of three-dimensional DNA nanostructures and potential biological applications.

Lo PK, Metera KL, Sleiman HF.

Curr. Opin. Chem. Biol. 2010 Oct;14(5):597-607. doi: 10.1016/j.cbpa.2010.08.002. Epub 2010 Sep 24. Review.

Stable gold nanoparticle conjugation to internal DNA positions: facile generation of discrete gold nanoparticle-DNA assemblies.

Wen Y, McLaughlin CK, Lo PK, Yang H, Sleiman HF.

Bioconjug. Chem. 2010 Aug 18;21(8):1413-6. doi: 10.1021/bc100160k.

Templated synthesis of DNA nanotubes with controlled, predetermined lengths.

Lo PK, Altvater F, Sleiman HF.

J. Am. Chem. Soc. 2010 Aug 4;132(30):10212-4. doi: 10.1021/ja1017442.

Long-range assembly of DNA into nanofibers and highly ordered networks using a block copolymer approach.

Carneiro KM, Aldaye FA, Sleiman HF.

J. Am. Chem. Soc. 2010 Jan 20;132(2):679-85. doi: 10.1021/ja907735m.

2009

Templated ligand environments for the selective incorporation of different metals into DNA.

Yang H, Rys AZ, McLaughlin CK, Sleiman HF.

Angew Chem Int Ed Engl. 2009;48(52):9919-23. doi: 10.1002/anie.200904186. No abstract available.

Metal-nucleic acid cages.

Yang H, McLaughlin CK, Aldaye FA, Hamblin GD, Rys AZ, Rouiller I, Sleiman HF.

Nat Chem. 2009 Aug;1(5):390-6. doi: 10.1038/nchem.290. Epub 2009 Jul 24.

Modular construction of DNA nanotubes of tunable geometry and single- or double-stranded character.

Aldaye FA, Lo PK, Karam P, McLaughlin CK, Cosa G, Sleiman HF.

Nat Nanotechnol. 2009 Jun;4(6):349-52. doi: 10.1038/nnano.2009.72. Epub 2009 Apr 12.

Nucleobase-templated polymerization: copying the chain length and polydispersity of living polymers into conjugated polymers.

Lo PK, Sleiman HF.

J Am Chem Soc. 2009 Apr 1;131(12):4182-3. doi: 10.1021/ja809613n.

Evaluation of binding selectivities and affinities of platinum-based quadruplex interactive complexes by electrospray ionization mass spectrometry.

Pierce SE, Kieltyka R, Sleiman HF, Brodbelt JS.

Biopolymers. 2009 Apr;91(4):233-43. doi: 10.1002/bip.21130.

2008

Assembling materials with DNA as the guide.

Aldaye FA, Palmer AL, Sleiman HF.

Science. 2008 Sep 26;321(5897):1795-9. doi: 10.1126/science.1154533. Review.

A platinum supramolecular square as an effective G-quadruplex binder and telomerase inhibitor.

Kieltyka R, Englebienne P, Fakhoury J, Autexier C, Moitessier N, Sleiman HF.

J Am Chem Soc. 2008 Aug 6;130(31):10040-1. doi: 10.1021/ja8014023. Epub 2008 Jul 11.

Templated synthesis of highly stable, electroactive, and dynamic metal-DNA branched junctions.

Yang H, Sleiman HF.

Angew Chem Int Ed Engl. 2008;47(13):2443-6. doi: 10.1002/anie.200703741.

Platinum phenanthroimidazole complexes as G-quadruplex DNA selective binders.

Kieltyka R, Fakhoury J, Moitessier N, Sleiman HF.

Chemistry. 2008;14(4):1145-54.

2007

Modular access to structurally switchable 3D discrete DNA assemblies.

Aldaye FA, Sleiman HF.

J Am Chem Soc. 2007 Nov 7;129(44):13376-7. Epub 2007 Oct 16. No abstract available.

Guest-mediated access to a single DNA nanostructure from a library of multiple assemblies.

Aldaye FA, Sleiman HF.

J Am Chem Soc. 2007 Aug 22;129(33):10070-1. Epub 2007 Aug 1.

DNA-protein noncovalent cross-linking: ruthenium dipyridophenazine biotin complex for the assembly of proteins and gold nanoparticles on DNA templates.

Slim M, Durisic N, Grutter P, Sleiman HF.

Chembiochem. 2007 May 7;8(7):804-12.

Dynamic DNA templates for discrete gold nanoparticle assemblies: control of geometry, modularity, write/erase and structural switching.

Aldaye FA, Sleiman HF.

J Am Chem Soc. 2007 Apr 11;129(14):4130-1. Epub 2007 Mar 17. No abstract available.

Molecule-responsive block copolymer micelles.

Ishihara Y, Bazzi HS, Toader V, Godin F, Sleiman HF.

Chemistry. 2007;13(16):4560-70.

pre-2007

Sequential self-assembly of a DNA hexagon as a template for the organization of gold nanoparticles.

Aldaye FA, Sleiman HF.

Angew Chem Int Ed Engl. 2006 Mar 27;45(14):2204-9. No abstract available.

Hydrogen-bond self-assembly of DNA-analogues into hexameric rosettes.

Rakotondradany F, Palmer A, Toader V, Chen B, Whitehead MA, Sleiman HF.

Chem Commun (Camb). 2005 Nov 21;(43):5441-3. Epub 2005 Oct 4.

Self-assembly of cyclic metal-DNA nanostructures using ruthenium tris(bipyridine)-branched oligonucleotides. Mitra D, Di Cesare N, Sleiman HF. Angew Chem Int Ed Engl. 2004 Nov 5;43(43):5804-8. Ruthenium(II)-phenanthroline-biotin complexes: synthesis and luminescence enhancement upon binding to avidin. Slim M, Sleiman HF. Bioconjug Chem. 2004 Sep-Oct;15(5):949-53. Ruthenium(II) dipyridoquinoxaline-norbornene: synthesis, properties, crystal structure, and use as a ROMP monomer. Rezvani A, Bazzi HS, Chen B, Rakotondradany F, Sleiman HF. Inorg Chem. 2004 Aug 9;43(16):5112-9. Photoresponsive supramolecular systems: self-assembly of azodibenzoic acid linear tapes and cyclic tetramers. Rakotondradany F, Whitehead MA, Lebuis AM, Sleiman HF. Chemistry. 2003 Oct 6;9(19):4771-80. Solid-Phase Synthesis of Transition Metal Linked, Branched Oligonucleotides This work was supported by NSERC (Canada), CFI (Canada) and FCAR (Quebec). The authors gratefully acknowledge Prof. M. J. Damha and his laboratory, McGill University, for helpful discussion. Vargas-Baca I, Mitra D, Zulyniak HJ, Banerjee J, Sleiman HF. Angew Chem Int Ed Engl. 2001 Dec 17;40(24):4629-4632.

Sleiman Lab Publications

2024

Control of the Assembly and Disassembly of Spherical Nucleic Acids is Critical for Enhanced Gene Silencing

DNA-Mediated Peptide Assembly into Protein Mimics

Automated Synthesis of Wireframe DNA Nanotubes

2023

Impact of the Core Chemistry of Self-Assembled Spherical Nucleic Acids on their In Vitro Fate

Dendritic amphiphilic siRNA: Selective albumin binding, in vivo efficacy and low toxicity

Minimalist Design of Wireframe DNA Nanotubes: Tunable Geometry, Size, Chirality, and Dynamics

Supramolecular Nucleic Acid-Based Organosilica Nanoparticles Responsive to Physical and Biological Inputs

Responsive Nucliec Acid-Based Organosilica Nanoparticles

Ultrabright DNA nanostructures for biosensing

DNA-encoded functionalized apatamers

Two-Dimensional Supramolecular Polymerization of DNA Amphiphiles is Driven by Sequence-Dependent DNA-Chromophore Interactions

Sequence-Controlled Spherical Nucleic Acids: Gene Silencing, Encapsulation, and Cellular Uptake

A Single Monomer Difference can Impact the Nanostructure Output of Precision Oligo(phosphodiesters)

Small Molecule-Templated DNA Hydrogel with Record Stiffness Integrates and Releases DNA Nanostructures and Gene Silencing Nucleic Acids

Stabilization of Functional DNA Structures with Mild Photochemical Methods

Programming rigidity into size-defined wireframe DNA nanotube

2022

DNA Sequence and Length Dictate the Assembly of Nucleic Acid Block Copolymers

Using transient equilibria (TREQ) to measure the thermodynamics of slowly assembling supramolecular systems

Asymmetric patterning drives the folding of a tripodal DNA nanotweezer

2021

Tuning DNA Supramolecular Polymers by the Addition of Small, Functionalized Nucleobase Mimics

Thermosetting supramolecular polymerization of compartmentalized DNA fibers with stereo sequence and length control

DNA Nanostructures: Current Challenges and Opportunities for Cellular Delivery

A dissipative pathway for the structural evolution of DNA fibres

Design and enhanced gene silencing activity of spherical 2′-fluoroarabinose nucleic acids (FANA-SNAs)

2020

A poly(thymine)–melamine duplex for the assembly of DNA nanomaterials

Molecular Printing with DNA Nanotechnology

Single-molecule methods in structural DNA nanotechnology

Amplified Self‐Immolative Release of Small Molecules by Spatial Isolation of Reactive Groups on DNA‐Minimal Architectures

Selection of a metal ligand modified DNAzyme for detecting Ni2+

Detailed cellular assessment of albumin-bound oligonucleotides: Increased stability and lower non-specific cell uptake

Target Self‐enhanced Selectivity in Metal‐specific DNAzymes

The assemble, grow and lift-off (AGLO) strategy to construct complex gold nanostructures with pre-designed morphologies

Sequence-Defined DNA Amphiphiles for Drug Delivery: Synthesis and Self-Assembly

Transition Metal‐Functionalized DNA Double‐Crossover Tiles: Enhanced Stability and Chirality Transfer to Metal Centers

2019

Advancing Wireframe DNA Nanostructures Using Single-Molecule Fluorescence Microscopy Techniques

Bottom‐Up Characterization and Self‐Assembly of Electrogenerated Chemiluminescence Active Ruthenium Nanospheres

Charting a course for chemistry

Spatial Presentation of Cholesterol Units on a DNA Cube as a Determinant of Membrane Protein-Mimicking Functions

Remote control of charge transport and chiral induction along a DNA-metallohelicate

Uptake and Fate of Fluorescently Labeled DNA Nanostructures in Cellular Environments: A Cautionary Tale

Minimalist Design of a Stimuli-Responsive Spherical Nucleic Acid for Conditional Delivery of Oligonucleotide Therapeutics

“Printing” DNA Strand Patterns on Small Molecules with Control of Valency, Directionality, and Sequence

Design Strategy to Access siRNA-Encapsulating DNA “Nanosuitcases” That Can Conditionally Release Their Cargo

2018

Kinetics of Strand Displacement and Hybridization on Wireframe DNA Nanostructures: Dissecting the Roles of Size, Morphology, and Rigidity

Templated synthesis of spherical RNA nanoparticles with gene silencing activity

Modular Strategy To Expand the Chemical Diversity of DNA and Sequence-Controlled Polymers

DNA-imprinted polymer nanoparticles with monodispersity and prescribed DNA-strand patterns

Mapping the energy landscapes of supramolecular assembly by thermal hysteresis

DNA Nanostructures at the Interface with Biology

Cyanine-mediated DNA nanofiber growth with controlled dimensionality

Recent Advances in DNA Nanotechnology

Encapsulation of gold nanoparticles into DNA minimal nanocages for complex anisotropic functionalization and assembly

Single-stranded templates as railroad tracks for hierarchical assembly of DNA origami

2017

DNA nanotechnology

Efficient and Rapid Mechanochemical Assembly of Platinum(II) Squares for Guanine Quadruplex Targeting

Long-Range Ordering of Blunt-Ended DNA tiles on Supported Lipid Bilayers

Stoichiometry and dispersity of DNA nanostructures using photobleaching pair correlation analysis

Precision Spherical Nucleic Acids for Delivery of Anticancer Drugs

Development of DNA nanostructures for high affinity binding to human serum albumin

Supramolecular DNA Nanotechnology

Cuvette‐Based Electrogenerated Chemiluminescence Detection System for the Assessment of Polymerizable Ruthenium Luminophores

2016

Optimized DNA “Nanosuitcases” for Encapsulation and Conditional Release of siRNA

DNA micelles as nanoreactors: efficient DNA functionalization with hydrophobic organic molecules

Minimalist Approach to Complexity: Templating the Assembly of DNA Tile Structures with Sequentially Grown Input Strands

“DNA–Teflon” sequence-controlled polymers

Synergy of Two Assembly Languages in DNA Nanostructures: Self-Assembly of Sequence-Defined Polymers on DNA Cages

Reprogramming the assembly of unmodified DNA with a small molecule

Transfer of molecular recognition information from DNA nanostructures to gold nanoparticles

2015

Dynamic DNA Nanotubes: Reversible Switching between Single and Double-Stranded Forms, and Effect of Base Deletions